FKBP2 Monoklonaler Antikörper

FKBP2 Monoklonal Antikörper für WB, IHC, ELISA, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2H7E8

Kat-Nr. : 66091-1-Ig

Synonyme

FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 2H7E8

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  WB analysis of human cerebellum using 66091-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66091-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 66091-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66091-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66091-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66091-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 66091-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug FKBP2 Monoclonal antibody (66091-1-Ig, Clone:2H7E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human cerebellum tissue, humanes Hirngewebe, Hausschwein-Lebergewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66091-1-Ig bindet in WB, IHC, FC (Intra), ELISA FKBP2 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FKBP2 fusion protein Ag19113
• Vollständiger Name: FK506 binding protein 2, 13kDa
• Berechnetes Molekulargewicht: 142 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC003384
• Gene symbol: FKBP2
• Gene ID (NCBI): 2286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).

Publikationen

Species	Application	Title
human	WB	Emerg Microbes Infect; Pharmacological suppression of glycogen synthase kinase-3 reactivates HIV-1 from latency via activating Wnt/β-catenin/TCF1 axis in CD4+ T cells.; Authors - Jing Wen
		mBio; Tryptophan Metabolism Activates Aryl Hydrocarbon Receptor-Mediated Pathway To Promote HIV-1 Infection and Reactivation.; Authors - Yan-Heng Zhou