FLCN Polyklonaler Antikörper

FLCN Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 11236-2-AP

Synonyme

BHD, Birt-Hogg-Dube syndrome protein, FLCL, Folliculin

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  WB analysis using 11236-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11236-2-AP (FLCN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 11236-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11236-2-AP (FLCN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, NCCIT-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11236-2-AP bindet in WB, IHC, IF, IP, ELISA FLCN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FLCN fusion protein Ag1746
• Vollständiger Name: folliculin
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 64-75 kDa
• GenBank-Zugangsnummer: BC015687
• Gene symbol: FLCN
• Gene ID (NCBI): 201163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLCN, also named as BHD, belongs to the folliculin family. It may play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). FLCN may be a tumor suppressor. It is involved in colorectal tumorigenesis. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. Mutation of FLCN may cause the BHD(Birt-Hogg-Dube syndrome ) or (PSP) primary spontaneous pneumothorax. And it may be involved in renal cell carcinoma.

Publikationen

Species	Application	Title
mouse	WB,IP	FASEB J; AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR.; Authors - Caterina Collodet
mouse	WB	DNA Repair (Amst); Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA.; Authors - Lim Teik How TH ; KD Validated
human	IHC	Front Mol Biosci; FLCN Regulates HIF2α Nuclear Import and Proliferation of Clear Cell Renal Cell Carcinoma.; Authors - Xuyang Zhao
	WB	J Trace Elem Med Biol; Methionine-chelated Zn promotes anabolism by integrating mTOR signal and autophagy pathway in juvenile yellow catfish.; Authors - Kun Wu
human	WB	Biochem Biophys Res Commun; Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O?-methylguanine-induced apoptosis.; Authors - Sano Shiori S ; KD Validated
rat	WB	Curr Res Neurobiol; Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models; Authors - Mira Seidel