FLVCR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FLVCR1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 26841-1-AP

Synonyme

Heme transporter FLVCR1, FLVCR, Feline leukemia virus subgroup C receptor-related protein 1

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  WB analysis of Jurkat using 26841-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 26841-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26841-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26841-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 26841-1-AP

  IP result of anti-FLVCR1 (IP:26841-1-AP, 4ug; Detection:26841-1-AP 1:500) with K-562 cells lysate 4400 ug.

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  IHC staining of human breast cancer using 26841-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26841-1-AP (FLVCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 26841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26841-1-AP (FLVCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 26841-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using 26841-1-AP (FLVCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

26841-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA FLVCR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FLVCR1 fusion protein Ag25220
• Vollständiger Name: feline leukemia virus subgroup C cellular receptor 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa~70 kDa
• GenBank-Zugangsnummer: BC048312
• Gene symbol: FLVCR1
• Gene ID (NCBI): 28982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLVCR1(feline leukemia virus subgroup C receptor 1) has been reported to have a crucial role in a variety of biological processes, including cell proliferation, cell death, apoptosis, oxidative stress response, cellular differentiation, and metabolism (PMID: 29532854). In addition, FLVCR1 can be applied as a clinical prognostic marker for patients with ESCC (PMID: 33842377).

Publikationen

Species	Application	Title
mouse	WB	J Nanobiotechnology; Apoptotic metabolites ameliorate bone aging phenotypes via TCOF1/FLVCR1-mediated mitochondrial homeostasis; Authors - Yan Qu ; KD Validated
		Cell Rep; Flvcr1a deficiency promotes heme-based energy metabolism dysfunction in skeletal muscle; Authors - Miriam Mistretta
human,mouse	WB,IP,IF	Cell Rep Med; Dysregulation of FLVCR1a-dependent mitochondrial calcium handling in neural progenitors causes congenital hydrocephalus; Authors - Francesca Bertino ; KD Validated
human	WB	Biochim Biophys Acta Mol Basis Dis; The role of FLVCR1 and FLVCR2 in choline transport in the Caco-2 intestinal epithelial cell model and rat small intestine; Authors - Tomoya Yasujima