Fibronectin Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Fibronectin Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(151)

CloneNo.

1G10F9

Rezensionen (4)

Kat-Nr. : 66042-1-Ig

Synonyme

FN1, 1G10F9, Anastellin, CIG, Cold insoluble globulin

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• 

  WB analysis of human plasma using 66042-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66042-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 66042-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 66042-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MG-63 using 66042-1-Ig

  MG-63 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat serum using 66042-1-Ig

  rat serum were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 66042-1-Ig

  IP result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug.

• 

  IHC staining of human colon cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 66042-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon cancer using 66042-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of NIH/3T3 using 66042-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of NIH/3T3 using 66042-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol ) fixed NIH/3T3 cells using 66042-1-Ig(Fibronectin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, HepG2-Zellen, HuH-7-Zellen, U2OS-Zellen
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Kolonkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 97 publications below
• IHC: See 43 publications below
• IF: See 44 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

66042-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment Fibronectin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fibronectin fusion protein Ag8016
• Vollständiger Name: fibronectin 1
• Berechnetes Molekulargewicht: 2386 aa, 263 kDa
• Beobachtetes Molekulargewicht: 263 kDa
• GenBank-Zugangsnummer: BC005858
• Gene symbol: Fibronectin
• Gene ID (NCBI): 2335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Publikationen

Species	Application	Title
mouse	WB	Protein Cell; Neutrophil extracellular traps license macrophage production of chemokines to facilitate CD8+ T cell infiltration in obstruction-induced renal fibrosis; Authors - Hongshuai Jia
human	IF	Dev Cell; Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages; Authors - Xinyi Huang
rat	WB,IF	Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi
human	WB,IF	Biomed Pharmacother; Nintedanib prevents TGF-β2-induced epithelial-mesenchymal transition in retinal pigment epithelial cells; Authors - Yiwei Yin
mouse	IHC	Oxid Med Cell Longev; HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis; Authors - Baopeng Xing
mouse	IHC	Front Immunol; Integrated analysis reveals crosstalk between pyroptosis and immune regulation in renal fibrosis; Authors - Fengxia Bai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (04-19-2023)

Worked well for IP, band seen just below 250 kD (page ruler plus ladder). Lower band is light chain IgG

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: BM-MSCs

Fibronectin Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in BM-MSCs (Cat no:66042-1-Ig)

FH

Ren Jie (Verified Customer) (07-11-2022)

Quite significant smear and non-specific binding

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human imortalized mammary fibroblast

Fibronectin Antibody Western Blot validation (1:1000 dilution) in Human imortalized mammary fibroblast (Cat no:66042-1-Ig)

FH

Deqiang (Verified Customer) (05-14-2020)

it works well on mouse embryo paraffin sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100

FH

Jihyun (Verified Customer) (05-07-2020)

There is no background signal. I can get a good one band in the WB. Great!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse epithelial cell