FNTB Monoklonaler Antikörper

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• KD/KO Validated

FNTB Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1F3B10

Kat-Nr. : 66783-1-Ig

Synonyme

1F3B10, EC:2.5.1.58, FPTB, FTase beta, FTase-beta

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  WB analysis of HeLa using 66783-1-Ig

  WB result of FNTB antibody (66783-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected HeLa cells.

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  WB analysis using 66783-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66783-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66783-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 66783-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FNTB antibody (66783-1-Ig, Clone: 1F3B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of A431 using 66783-1-Ig

  1x10^6 A431 cells were intracellularly stained with 0.8 ug FNTB Monoclonal antibody (66783-1-Ig, Clone:1F3B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66783-1-Ig bindet in WB, IF/ICC, FC (Intra), ELISA FNTB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FNTB fusion protein Ag27796
• Vollständiger Name: farnesyltransferase, CAAX box, beta
• Berechnetes Molekulargewicht: 437 aa, 49 kDa
• Beobachtetes Molekulargewicht: 44-45 kDa
• GenBank-Zugangsnummer: BC020232
• Gene symbol: FNTB
• Gene ID (NCBI): 2342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.