PSMA/GCPII Monoklonaler Antikörper

PSMA/GCPII Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

3G4E12

Kat-Nr. : 66678-1-Ig

Synonyme

FOLH1, PSMA, 3G4E12, Cell growth-inhibiting gene 27 protein, EC:3.4.17.21

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  WB analysis of LNCaP using 66678-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66678-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 66678-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66678-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66678-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66678-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66678-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66678-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human prostate cancer using 66678-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-3 using 66678-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:90 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-3 using 66678-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  Affinity and Kinetic Characterization of 66678-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 66678-1-Ig against Human PSMA/GCPII was performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe, LNCaP-Zellen, Maushirngewebe, Mausnierengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Prostatakarzinomgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66678-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA PSMA/GCPII und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMA/GCPII fusion protein Ag16594
• Vollständiger Name: folate hydrolase (prostate-specific membrane antigen) 1
• Berechnetes Molekulargewicht: 719 aa, 81 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC025672
• Gene symbol: PSMA
• Gene ID (NCBI): 2346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Sci Rep; Cancer cell's neuroendocrine feature can be acquired through cell-cell fusion during cancer-neural stem cell interaction.; Authors - Liyuan Yin
human	WB	Am J Clin Exp Urol; Transmembrane prostatic acid phosphatase: a therapeutic target in advanced prostate cancer; Authors - Alexander Kirschenbaum
human		RSC Med Chem; Design, synthesis and biological evaluation of a novel PSMA-PI3K small molecule drug conjugate; Authors - Shouguo Peng