FOXO1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FOXO1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(175)

Rezensionen (2)

Kat-Nr. : 18592-1-AP

Synonyme

FKH 1, FKH1, FKHR, forkhead box O1, Forkhead box protein O1

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• 

  WB analysis using 18592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18592-1-AP (FOXO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 18592-1-AP

  IP result of anti-FOXO1 (IP:18592-1-AP, 4ug; Detection:18592-1-AP 1:2000) with HepG2 cells lysate 960 ug.

• 

  IHC staining of human kidney using 18592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18592-1-AP (FOXO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18592-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FOXO1 antibody (18592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Daudi using 18592-1-AP

  1x10^6 Daudi cells were intracellularly stained with 0.4 ug FOXO1 Polyclonal antibody (18592-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HEK-293T-Zellen, HepG2-Zellen, Maushirngewebe, Mausnierengewebe, Rattenhirngewebe, SGC-7901-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Daudi-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 161 publications below
• IHC: See 16 publications below
• IF: See 27 publications below
• IP: See 4 publications below
• ChIP: See 4 publications below

Produktinformation

18592-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA FOXO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXO1 fusion protein Ag13296
• Vollständiger Name: forkhead box O1
• Berechnetes Molekulargewicht: 655 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC021981
• Gene symbol: FOXO1
• Gene ID (NCBI): 2308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).

Publikationen

Species	Application	Title
mouse	WB	J Exp Med; PD-1 and TIM-3 differentially regulate subsets of mouse IL-17A–producing γδ T cells; Authors - Sarah C Edwards
human	WB,ChIP	Cell Res; Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.; Authors - Yanjing Guo
mouse	IF	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
mouse	WB	Redox Biol; miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation; Authors - Xiaofei Wang
mouse	WB,IF	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
mouse	IHC	Toxicology; Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice; Authors - Yong-Jiang Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hala (Verified Customer) (01-27-2023)

an specific band is observed around 20 KDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/4000
• Cell Tissue Type: hepg2

FOXO1 Antibody Western Blot validation (1/4000 dilution) in hepg2 (Cat no:18592-1-AP)

FH

Ruchi (Verified Customer) (07-08-2022)

I used the free sample antibody for immunofluorescence and I am happy with the results. I would like to order Proteintech products for my lab needs

• Applications: Immunofluorescence
• Primary Antibody Dilution: FOXO1
• Cell Tissue Type: Mouse skin

FOXO1 Antibody Immunofluorescence validation (FOXO1 dilution) in Mouse skin (Cat no:18592-1-AP)