FREM1 Polyklonaler Antikörper

FREM1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13086-1-AP

Synonyme

FRAS1-related extracellular matrix protein 1, C9orf154, C9orf145, C9orf143

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  WB analysis of human plasma using 13086-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 13086-1-AP (FREM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HepG2 using 13086-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13086-1-AP (FREM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human liver cancer using 13086-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13086-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 13086-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 13086-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13086-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FREM1 antibody (13086-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

13086-1-AP bindet in WB, IHC, IF/ICC, ELISA FREM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FREM1 fusion protein Ag3617
• Vollständiger Name: FRAS1 related extracellular matrix 1
• Berechnetes Molekulargewicht: 2179aa, 244 kDa, 149.3 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa, 70-80 kDa
• GenBank-Zugangsnummer: BC031064
• Gene symbol: FREM1
• Gene ID (NCBI): 158326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FREM1, C9orf143, C9orf145, C9orf154 and QBRICK, is an extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. This antibody recognizes isoform 1 (244 kDa), isform 2 (80 kDa) and isoform 3 (43 kDa).

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); TILRR (FREM1 isoform 2) is a prognostic biomarker correlated with immune infiltration in breast cancer.; Authors - Xiao-Yi Xu
human	IHC	Cancer Med; Elevated expression of FREM1 in breast cancer indicates favorable prognosis and high-level immune infiltration status.; Authors - Han-Ning Li