FUCA1 Polyklonaler Antikörper

FUCA1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16420-1-AP

Synonyme

Alpha L fucosidase 1, Alpha L fucosidase I, FUCA, FUCA1, fucosidase, alpha L 1, tissue, Tissue alpha L fucosidase

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 16420-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16420-1-AP (FUCA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16420-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16420-1-AP (FUCA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 4 publications below

Produktinformation

16420-1-AP bindet in WB, IHC, ELISA FUCA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FUCA1 fusion protein Ag9612
• Vollständiger Name: fucosidase, alpha-L- 1, tissue
• Berechnetes Molekulargewicht: 461 aa, 53 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC017338
• Gene symbol: FUCA1
• Gene ID (NCBI): 2517
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).

Publikationen

Species	Application	Title
human	WB,IHC	Oncotarget; Reduced expression of α-L-Fucosidase-1 (FUCA-1) predicts recurrence and shorter cancer specific survival in luminal B LN+ breast cancer patients.; Authors - Serena Bonin
human	WB,IHC	Oncotarget; Human a-L-fucosidase-1 attenuates the invasive properties of thyroid cancer.; Authors - Giancarlo Vecchio
human	WB	Cancer Genomics Proteomics; High Resolution Proteomic Analysis of the Cervical Cancer Cell Lines Secretome Documents Deregulation of Multiple Proteases.; Authors - Kalliopi I Pappa
human	IHC	Pathol Int; Alpha-L-fucosidase-1 is a diagnostic marker that distinguishes mucoepidermoid carcinoma from squamous cell carcinoma.; Authors - Shoko Ishida
human	IHC	Oncotarget; Proteomics analysis of bladder cancer invasion: Targeting EIF3D for therapeutic intervention.; Authors - Agnieszka Latosinska
human		Oncogenesis; USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer; Authors - Yi Xiao