FUCA1 Monoklonaler Antikörper

FUCA1 Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1G5C5

Kat-Nr. : CL594-67251

Synonyme

FUCA, EC:3.2.1.51, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase I, Alpha-L-fucosidase 1

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  IF Staining of human liver cancer using CL594-67251

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 FUCA1 antibody (CL594-67251, Clone: 1G5C5 ) at dilution of 1:200. DAPI (blue).

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  IF Staining of human liver cancer using CL594-67251

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 FUCA1 antibody (CL594-67251, Clone: 1G5C5 ) at dilution of 1:200. DAPI (blue).

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  IF Staining of HepG2 using CL594-67251

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 FUCA1 antibody (CL594-67251, Clone: 1G5C5 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67251 bindet in IF/ICC, IF-P FUCA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FUCA1 fusion protein Ag9638
• Vollständiger Name: fucosidase, alpha-L- 1, tissue
• Berechnetes Molekulargewicht: 461 aa, 53 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC017338
• Gene symbol: FUCA1
• Gene ID (NCBI): 2517
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).