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FXR1 Monoklonaler Antikörper

FXR1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E12E8

Kat-Nr. : CL647-67813

Synonyme

FXR1, hFXR1p

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated FXR1 Monoclonal anti (CL647-67813)

    IF Staining of mouse brain using CL647-67813

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 FXR1 antibody (CL647-67813, Clone: 1E12E8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated FXR1 Monoclonal anti (CL647-67813)

    FC experiment of HeLa using CL647-67813

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human FXR1 (CL647-67813, Clone:1E12E8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaushirngewebe
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-67813 bindet in IF, FC (Intra) FXR1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen FXR1 fusion protein Ag27800
Vollständiger Name fragile X mental retardation, autosomal homolog 1
Berechnetes Molekulargewicht 621 aa, 70 kDa
Beobachtetes Molekulargewicht70-80 kDa
GenBank-ZugangsnummerBC028983
Gene symbol FXR1
Gene ID (NCBI) 8087
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MV 69 kDa and 60 kDa.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 647 FXR1 antibody CL647-67813Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen