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FXYD6 Polyklonaler Antikörper
FXYD6 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 15805-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | PC-12-Zellen, humanes Hirngewebe, humanes Hodengewebe, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen |
Erfolgreiche Detektion in IHC | humanes Hirngewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | PC-12-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunhistochemie (IHC) | IHC : 1:20-1:200 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 3 publications below |
IF | See 2 publications below |
Produktinformation
15805-1-AP bindet in WB, IHC, IF/ICC, ELISA FXYD6 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | FXYD6 fusion protein Ag8538 |
Vollständiger Name | FXYD domain containing ion transport regulator 6 |
Berechnetes Molekulargewicht | 95 aa, 11 kDa |
Beobachtetes Molekulargewicht | 20 kDa |
GenBank-Zugangsnummer | BC018652 |
Gene symbol | FXYD6 |
Gene ID (NCBI) | 53826 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The FXYD family is a group of small single-span transmembrane proteins characterized by a signature sequence containing an FXYD motif, two conserved glycines and a serine residue. Members of the FXYD family, including FXYD1 (phospholemman), FXYD2 (gamma subunit of Na,K-ATPase), FXYD3 (Mat8), FXYD4 (CHIF), FXYD5 (RIC), FXYD6 (phosphohippolin) and FXYD7, are tissue specific regulators of the Na,K-ATPase. FXYD6 is primarily expressed in the brain. It modulates the kinetic activity of Na,K-ATPase and has long-term physiological importance in maintaining cation homeostasis. It may play a role in endolymph composition and has a potential important role in neuronal excitability of the CNS during postnatal development and in the adult brain. On the SDS-PAGE FXYD6 migrates with an apparent molecular weight of approximately 20 kDa, which is larger than the calculated molecular weight of 10.5 kDa (PMID: 15193427; 17209044). The gene encodes FXYD6 is located on chromosome 11q23.3, and it might be a susceptibility gene of schizophrenia.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for FXYD6 antibody 15805-1-AP | Protokoll herunterladen |
IHC protocol for FXYD6 antibody 15805-1-AP | Protokoll herunterladenl |
IF protocol for FXYD6 antibody 15805-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
J Am Soc Nephrol Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation. | ||
Cancer Med Identification of FXYD6 as the novel biomarker for glioma based on differential expression and DNA methylation | ||
Biomed Res Int FXYD6 Regulates Chemosensitivity by Mediating the Expression of Na+/K+-ATPase α1 and Affecting Cell Autophagy and Apoptosis in Colorectal Cancer.
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Front Immunol Identification of prognostic subtypes and the role of FXYD6 in ovarian cancer through multi-omics clustering |