Fascin Monoklonaler Antikörper

Fascin Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5G3

Kat-Nr. : CL488-66321

Synonyme

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FLJ38511, FSCN1, HSN, p55, Singed like protein, SNL

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Galerie der Validierungsdaten

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  IF Staining of human tonsillitis using CL488-66321

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66321 (Fascin antibody) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL488-66321

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66321 (Fascin antibody) at dilution of 1:100. .

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  FC experiment of SH-SY5Y using CL488-66321

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Fascin (CL488-66321, Clone:1B5G3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using CL488-66321

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Fascin (CL488-66321, Clone:1B5G3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66321 bindet in IF, FC (Intra) Fascin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fascin fusion protein Ag6074
• Vollständiger Name: fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC000521
• Gene symbol: FSCN1
• Gene ID (NCBI): 6624
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C