Fascin Monoklonaler Antikörper

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• KD/KO Validated

Fascin Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1B5G3

Kat-Nr. : CL594-66321

Synonyme

FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein

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  IF Staining of human tonsillitis using CL594-66321

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66321 (Fascin antibody) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL594-66321

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66321 (Fascin antibody) at dilution of 1:100.

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  FC experiment of HeLa using CL594-66321

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human Fascin (CL594-66321, Clone:1B5G3) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66321 bindet in IF-P, FC (Intra) Fascin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fascin fusion protein Ag6074
• Vollständiger Name: fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Berechnetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC000521
• Gene symbol: Fascin
• Gene ID (NCBI): 6624
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.