Fibrillin 1 Polyklonaler Antikörper

Fibrillin 1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 29425-1-AP

Synonyme

FBN1, Asprosin, FBN, Fibrillin-1, MASS

---

• 

  WB analysis using 29425-1-AP

  HeLa cells were subjected to Tris-acetate gel system followed by western blot with 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 29425-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 29425-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 29425-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 29425-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Fibrillin 1 antibody (29425-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe, human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

29425-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA Fibrillin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fibrillin 1 fusion protein Ag30283
• Vollständiger Name: fibrillin 1
• Beobachtetes Molekulargewicht: 350 kDa
• GenBank-Zugangsnummer: BC146854
• Gene symbol: Fibrillin 1
• Gene ID (NCBI): 2200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrillin 1 is a 350 kDa large extracellular matrix glycoprotein that formed thread-like microfibril filaments, which provided structural support for tissues, formed elastic fibers in the skin and vessels, regulated the availability of the transforming growth factor beta (TGF-β) protein, and modulated the microenvironment. Fibrillin 1 contains a large number of cysteine-rich epidermal growth factor (EGF)- like domains, most capable of binding calcium ions (cbEGF-like), and a few motifs homologous to the binding protein for transforming growth factor beta (TGFβ1-BP)- like module (PMID: 29220879, PMID: 35234370).

Publikationen

Species	Application	Title
mouse	WB,CoIP	Cells; ADAR1 Is Essential for Smooth Muscle Homeostasis and Vascular Integrity; Authors - Dunpeng Cai