Flotillin 1 Monoklonaler Antikörper

Flotillin 1 Monoklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1D6C6

Kat-Nr. : 67968-1-Ig

Synonyme

FLOT1, Flotillin-1, 1D6C6

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  WB analysis using 67968-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 67968-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67968-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67968-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67968-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Raji using 67968-1-Ig

  1x10^6 Raji cells were intracellularly stained with 0.2 ug Flotillin 1 Monoclonal antibody (67968-1-Ig, Clone:1D6C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Jurkat-Zellen, MOLT-4-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Raji-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in FC (Intra): Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67968-1-Ig bindet in WB, FC (Intra), ELISA Flotillin 1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Flotillin 1 fusion protein Ag8694
• Vollständiger Name: flotillin 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC001146
• Gene symbol: Flotillin 1
• Gene ID (NCBI): 10211
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.