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G-CSF Polyklonaler Antikörper

G-CSF Polyklonal Antikörper für WB, ELISA, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA, FC (Intra)

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17185-1-AP

Synonyme

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen, MOLT-4-Zellen
Erfolgreiche Detektion in FC (Intra)K-562-Zellen
Erfolgreiche Detektion in FCK-562-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17185-1-AP bindet in WB, IHC, ELISA, FC (Intra) G-CSF und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen G-CSF fusion protein Ag10968
Vollständiger Name colony stimulating factor 3 (granulocyte)
Berechnetes Molekulargewicht 200 aa, 22 kDa
Beobachtetes Molekulargewicht20 kDa
GenBank-ZugangsnummerBC033245
Gene symbol G-CSF
Gene ID (NCBI) 1440
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for G-CSF antibody 17185-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Mol Immunol

Granulocyte colony stimulating factor (G-CSF) regulates neutrophils infiltration and periodontal tissue destruction in an experimental periodontitis.

Authors - Zheng Zhang
humanWB,IHC

Int J Mol Sci

A NOTCH1 Mutation Found in a Newly Established Ovarian Cancer Cell Line (FDOVL) Promotes Lymph Node Metastasis in Ovarian Cancer

Authors - Wei Jiang
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