G-CSF Polyklonaler Antikörper

G-CSF Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17185-1-AP

Synonyme

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

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Galerie der Validierungsdaten

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  WB analysis using 17185-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17185-1-AP (G-CSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17185-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17185-1-AP (G-CSF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17185-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17185-1-AP (G-CSF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 17185-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human G-CSF (17185-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

17185-1-AP bindet in WB, IHC, FC, ELISA G-CSF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: G-CSF fusion protein Ag10968
• Vollständiger Name: colony stimulating factor 3 (granulocyte)
• Berechnetes Molekulargewicht: 200 aa, 22 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC033245
• Gene symbol: CSF3
• Gene ID (NCBI): 1440
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.

Publikationen

Species	Application	Title
human	IHC	Mol Immunol; Granulocyte colony stimulating factor (G-CSF) regulates neutrophils infiltration and periodontal tissue destruction in an experimental periodontitis.; Authors - Zheng Zhang
human	WB,IHC	Int J Mol Sci; A NOTCH1 Mutation Found in a Newly Established Ovarian Cancer Cell Line (FDOVL) Promotes Lymph Node Metastasis in Ovarian Cancer; Authors - Wei Jiang ; KD Validated