G6PD Monoklonaler Antikörper

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• KD/KO Validated

G6PD Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

2A7B12

Kat-Nr. : CL488-66373

Synonyme

Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6P, EC:1.1.1.49, 2A7B12

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  IF Staining of HeLa using CL488-66373

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 G6PD antibody (CL488-66373, Clone: 2A7B12 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-66373

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated G6PD Monoclonal antibody (CL488-66373, Clone:2A7B12)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66373 bindet in IF/ICC, FC (Intra) G6PD und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: G6PD fusion protein Ag21862
• Vollständiger Name: glucose-6-phosphate dehydrogenase
• Berechnetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC000337
• Gene symbol: G6PD
• Gene ID (NCBI): 2539
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
		Nat Commun; Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell death; Authors - Michal Scur