GAB3-Specific Polyklonaler Antikörper

GAB3-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20089-1-AP

Synonyme

GAB3, Growth factor receptor bound protein 2-associated protein 3, GRB2-associated-binding protein 3, GRB2-associated binder 3, GRB2 associated binder 3

---

• 

  WB analysis of mouse brain using 20089-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20089-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20089-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 20089-1-AP

  IP result of anti-GAB3-Specific (IP:20089-1-AP, 4ug; Detection:20089-1-AP 1:2000) with MCF-7 cells lysate 3200ug.

• 

  IHC staining of human liver cancer using 20089-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 20089-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 20089-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20089-1-AP (GAB3-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20089-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GAB3-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GRB2-associated binding protein 3
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: NM_001081573
• Gene symbol: GAB3
• Gene ID (NCBI): 139716
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAB3 belongs to the GAB family. GAB3 interacts with PIK3R/p85, SHP2 and GRAP2/MONA. It may interact with Grb2. The antibody is specific to GAB3.