GABARAPL1-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GABARAPL1-Specific Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11010

Synonyme

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GABARAPL1-Specific, GEC 1, GEC1

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL488-11010

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 488 GABARAPL1-Specific antibody (CL488-11010) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of HepG2 using CL488-11010

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 488 GABARAPL1-Specific antibody (CL488-11010) at dilution of 1:200, CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: mit Chloroquin behandelte HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11010 bindet in IF GABARAPL1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABARAPL1-Specific fusion protein Ag1473
• Vollständiger Name: GABA(A) receptor-associated protein like 1
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC009309
• Gene symbol: GABARAPL1
• Gene ID (NCBI): 23710
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine. The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.