GABARAPL2-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GABARAPL2-Specific Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 18724-1-AP

Synonyme

ATG8, FLC3A, GABARAPL2, GATE 16, GATE16, GEF 2, GEF2

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 18724-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18724-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18724-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 18724-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18724-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18724-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18724-1-AP (GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HepG2 using 18724-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug GABARAPL2-Specific antibody (18724-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, humanes Hirngewebe, Maushirngewebe, Mausmilzgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 2 publications below

Produktinformation

18724-1-AP bindet in WB, IHC, FC, ELISA GABARAPL2-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GABA(A) receptor-associated protein-like 2
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC005985
• Gene symbol: GABARAPL2
• Gene ID (NCBI): 11345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.

Publikationen

Species	Application	Title
mouse	WB	Nat Immunol; Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.; Authors - Miwa Sasai ; KO Validated
rat	WB	Autophagy; CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.; Authors - Hyun-Kyu An
human	WB	Autophagy; Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.; Authors - Jonathan A Stefely
human	IHC	Comput Biol Med; Identifying mitophagy-related genes as prognostic biomarkers and therapeutic targets of gastric carcinoma by integrated analysis of single-cell and bulk-RNA sequencing data; Authors - Zehua Wang
human	WB	Cell Death Dis; Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.; Authors - Christos E Zois
human	WB, IHC	Neurobiol Dis; Alteration of autophagosomal proteins in the brain of multiple system atrophy.; Authors - Tanji Kunikazu K

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