GABRA2 Rekombinanter Antikörper

GABRA2 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

230158E6

Kat-Nr. : 83057-2-RR

Synonyme

230158E6

---

• 

  WB analysis using 83057-2-RR

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83057-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 83057-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GABRA2 Recombinant antibody (83057-2-RR, Clone:230158E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83057-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83057-2-RR against Human GABRA2 were performed. The affinity constant is 0.403 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

83057-2-RR bindet in WB, FC (Intra), ELISA GABRA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GABRA2 fusion protein Ag29301
• Vollständiger Name: gamma-aminobutyric acid (GABA) A receptor, alpha 2
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-51 kDa
• GenBank-Zugangsnummer: NM_000807
• Gene symbol: GABRA2
• Gene ID (NCBI): 2555
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.

Publikationen

Species	Application	Title
mouse	WB	J Ethnopharmacol; Flavonoids from mulberry leaves exhibit sleep-improving effects via regulating GABA and 5-HT receptors; Authors - Rui Li
rat	WB	Int Immunopharmacol; Promotion of sleep by cinnamic acid in parachlorophenylalanine-induced insomnia in rats; Authors - Xiaona Ye