GABRA2 Rekombinanter Antikörper

GABRA2 Rekombinant Antikörper für WB, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra)

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230158E6

Kat-Nr. : CL750-83057-2

Synonyme

230158E6

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  WB analysis using CL750-83057-2

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83057-2 (GABRA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A549 using CL750-83057-2

  1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated GABRA2 Recombinant antibody (CL750-83057-2, Clone:230158E6)(red), or 4 ug Isotype Control (CL750-30000, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: unboiled rat brain tissue, Maus-Cerebellum-Gewebe, ungekochtes Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-83057-2 bindet in WB, FC (Intra) GABRA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GABRA2 fusion protein Ag29301
• Vollständiger Name: gamma-aminobutyric acid (GABA) A receptor, alpha 2
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-51 kDa
• GenBank-Zugangsnummer: NM_000807
• Gene symbol: GABRA2
• Gene ID (NCBI): 2555
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.