GAGE2D Polyklonaler Antikörper

GAGE2D Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12532-1-AP

Synonyme

GAGE-2D, GAGE 8, G antigen 8, G antigen 2D, CT4.8

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  WB analysis of K-562 using 12532-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12532-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 12532-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 12532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 12532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 12532-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12532-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 12532-1-AP

  Immunofluorescent analysis of HepG2 cells, using GAGE2D antibody 12532-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12532-1-AP bindet in WB, IHC, IF/ICC, ELISA GAGE2D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAGE2D fusion protein Ag3230
• Vollständiger Name: G antigen 2D
• Berechnetes Molekulargewicht: 116 aa, 13 kDa
• Beobachtetes Molekulargewicht: 18-22 kDa
• GenBank-Zugangsnummer: BC018052
• Gene symbol: GAGE2D
• Gene ID (NCBI): 729408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAGE2D, also named as GAGE8 and CT4.8, is expressed in testis and tumor tissues.