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Galectin-3 Monoklonaler Antikörper
Galectin-3 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IP, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1C1B2
Kat-Nr. : 60207-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A431-Zellen, A549-Zellen, Caco-2-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen, T-47D-Zellen |
| Erfolgreiche Detektion in IHC | humanes Schilddrüsenkarzinomgewebe, humanes Kolongewebe, humanes Ovarialkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | HeLa-Zellen, humanes Schilddrüsenkarzinomgewebe, Mausnierengewebe, MCF-7-Zellen |
| Erfolgreiche Detektion in FC | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
| Immunfluoreszenz (IF) | IF : 1:400-1:1600 |
| Durchflusszytometrie (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 2 publications below |
| WB | See 18 publications below |
| IHC | See 8 publications below |
| IF | See 14 publications below |
| IP | See 1 publications below |
Produktinformation
60207-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA Galectin-3 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2b |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Galectin-3 fusion protein Ag6753 |
| Vollständiger Name | lectin, galactoside-binding, soluble, 3 |
| Berechnetes Molekulargewicht | 26 kDa |
| Beobachtetes Molekulargewicht | 31 kDa |
| GenBank-Zugangsnummer | BC001120 |
| Gene symbol | LGALS3 |
| Gene ID (NCBI) | 3958 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for Galectin-3 antibody 60207-1-Ig | Protokoll herunterladen |
| IHC protocol for Galectin-3 antibody 60207-1-Ig | Protokoll herunterladen |
| IF protocol for Galectin-3 antibody 60207-1-Ig | Protokoll herunterladen |
| FC protocol for Galectin-3 antibody 60207-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cancer Res CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer | ||
Circ. Res. Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm. | ||
Acta Biomater Tumor cell membrane-based peptide delivery system targeting the tumor microenvironment for cancer immunotherapy and diagnosis. | ||
Front Immunol Single-cell RNA sequencing reveals a novel inhibitory effect of ApoA4 on NAFL mediated by liver-specific subsets of myeloid cells | ||
Theranostics Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis. | ||
Cell Rep Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (08-29-2023) | I used this antibody in mouse kidney tissues and it worked well.
|
FH Muhammad (Verified Customer) (09-13-2022) | Work best in HUVECs
|
FH Noémie (Verified Customer) (10-20-2021) | I used this antibody for Western Blot against Gal3 in mamalian cells (U2OS), and did a validation by siRNA.
 |
