GALE Polyklonaler Antikörper

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• KD/KO Validated

GALE Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 14414-1-AP

Synonyme

UDP-GlcNAc 4-epimerase, UDP-GalNAc 4-epimerase, UDP-galactose 4-epimerase, SDR1E1, EC:5.1.3.7

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  WB analysis of A375 using 14414-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14414-1-AP (GALE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14414-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14414-1-AP (GALE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14414-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14414-1-AP (GALE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14414-1-AP

  IP result of anti-GALE (IP:14414-1-AP, 4ug; Detection:14414-1-AP 1:300) with HeLa cells lysate 1080ug.

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  IHC staining of human malignant melanoma using 14414-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14414-1-AP (GALE antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human malignant melanoma using 14414-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14414-1-AP (GALE antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14414-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GALE antibody (14414-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

14414-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GALE und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GALE fusion protein Ag5756
• Vollständiger Name: UDP-galactose-4-epimerase
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC050685
• Gene symbol: GALE
• Gene ID (NCBI): 2582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).

Publikationen

Species	Application	Title
rat	WB	Redox Biol; Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.; Authors - Wei Ou
human	WB, IHC	PLoS One; Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.; Authors - Yinxian Wen
human	WB	Infect Drug Resist; iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae.; Authors - Yan Wang
human	WB, IHC	Biochem Biophys Res Commun; The critical role of UDP-galactose-4-epimerase in osteoarthritis: modulating proteoglycans synthesis of articular chondrocytes.; Authors - Yinxian Wen ; KD Validated