GAPDH (Human Specific) Rekombinanter Antikörper

GAPDH (Human Specific) Rekombinant Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

2G21

Kat-Nr. : 80570-1-RR

Synonyme

G3PD, GAPD, GAPDH, OK/SW cl.12

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Galerie der Validierungsdaten

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  WB analysis using 80570-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80570-1-RR (GAPDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 80570-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80570-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 80570-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 80570-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 80570-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 80570-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

80570-1-RR bindet in WB, IHC, IF, ELISA GAPDH (Human Specific) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GAPDH (Human Specific) fusion protein Ag0766
• Vollständiger Name: glyceraldehyde-3-phosphate dehydrogenase
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC004109
• Gene symbol: GAPDH
• Gene ID (NCBI): 2597
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.

Publikationen

Species	Application	Title
human	WB	Mater Today Bio; Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration.; Authors - Zhi-Hu Zhao
human	WB	Front Mol Biosci; SLC7A11, a Potential Therapeutic Target Through Induced Ferroptosis in Colon Adenocarcinoma.; Authors - Xin Cheng
human	WB	J Biochem Mol Toxicol; ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway; Authors - Guanli Zhang
	WB	Diagn Pathol; The multi-omics analyses of acsl1 reveal its translational significance as a tumor microenvironmental and prognostic biomarker in clear cell renal cell carcinoma; Authors - Yang Yang
human	WB	Medicine (Baltimore); Integrated analysis of potential biomarkers associated with diabetic periodontitis development based on bioinformatics: An observational study; Authors - Yiran Wu
human	WB	J Cancer Res Clin Oncol; High expression of cuproptosis-related gene FDX1 in relation to good prognosis and immune cells infiltration in colon adenocarcinoma (COAD); Authors - Lizong Wang