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GAPDH (Human Specific) Rekombinanter Antikörper

GAPDH (Human Specific) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2G21

Kat-Nr. : CL594-80570

Synonyme

G3PD, GAPD, GAPDH, OK/SW cl.12

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated GAPDH (Human Specific) Rec (CL594-80570)

    FC experiment of HeLa using CL594-80570

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GAPDH (Human Specific) (CL594-80570, Clone:2G21) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-80570 bindet in FC (Intra) GAPDH (Human Specific) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GAPDH (Human Specific) fusion protein Ag0766
Vollständiger Name glyceraldehyde-3-phosphate dehydrogenase
Berechnetes Molekulargewicht 36 kDa
GenBank-ZugangsnummerBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.

Protokolle

Produktspezifische Protokolle
FC protocol for CL594 GAPDH (Human Specific) antibody CL594-80570Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen