GATA6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GATA6 Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 55435-1-AP

Synonyme

GATA-binding factor 6, Transcription factor GATA 6, Transcription factor GATA-6

---

• 

  WB analysis of MCF-7 using 55435-1-AP

  WB result of GATA6 antibody (55435-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA6 transfected MCF-7 cells.

• 

  WB analysis using 55435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55435-1-AP (GATA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 55435-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55435-1-AP (GATA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HuH-7 using 55435-1-AP

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human GATA6 (55435-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, BxPC-3-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HuH-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

55435-1-AP bindet in WB, IHC, IF, FC (Intra), CoIP, ELISA GATA6 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GATA binding protein 6
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60-64 kDa
• GenBank-Zugangsnummer: NM_005257
• Gene symbol: GATA6
• Gene ID (NCBI): 2627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA6 is a member of the GATA family of zinc-finger transcriptional regulators, which play crucial roles in the regulation of cell growth, differentiation, survival, and maintenance of body functions. Named after the conserved base sequence (G/A)GATA(A/T), GATA6 features conserved tandem zinc fingers and is essential in coordinating the development and precise gene regulation of diverse tissues, including the heart and gastrointestinal tract. GATA6 recognizes the sequence (A/T/C)GATA(A/T)A and interacts with other transcriptional regulators through its zinc-finger domain.

Publikationen

Species	Application	Title
mouse	IHC	NPJ Precis Oncol; CCL3 predicts exceptional response to TGFβ inhibition in basal-like pancreatic cancer enriched in LIF-producing macrophages; Authors - Silvia Pietrobono
mouse	CoIP	Phytomedicine; Targeting GATA6 with pedunculoside inhibits fetal gene expression to attenuate pathological cardiac hypertrophy; Authors - An Pan
mouse	WB	Environ Sci Technol; Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling.; Authors - Zhihua Ren
mouse	WB,IF	iScience; Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency; Authors - Qian Ban
human	WB	FASEB J; Pulmonary expression of CYP2A13 and ABCB1 is regulated by FOXA2 and their genetic interaction is associated with lung cancer.; Authors - Chan Xiang
mouse	WB	Front Cell Dev Biol; ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.; Authors - XiaoYun Sun