GATC Monoklonaler Antikörper

GATC Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F9C8

Kat-Nr. : 68405-1-Ig

Synonyme

Protein 15E1.2, Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial, Glu-AdT subunit C, GatC like protein, EC:6.3.5.-

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  WB analysis of A549 using 68405-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68405-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 68405-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GATC antibody (68405-1-Ig, Clone: 2F9C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HEK-293 using 68405-1-Ig

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GATC Monoclonal antibody (68405-1-Ig, Clone:2F9C8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A549-Zellen, HEK-293-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68405-1-Ig bindet in WB, IF/ICC, ELISA GATC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GATC fusion protein Ag19857
• Vollständiger Name: glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
• Berechnetes Molekulargewicht: 136 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC107145
• Gene symbol: GATC
• Gene ID (NCBI): 283459
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).