GBP3 Rekombinanter Antikörper

GBP3 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

230178D8

Kat-Nr. : 83534-1-RR

Synonyme

GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3, EC:3.6.5.-

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  WB analysis using 83534-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83534-1-RR (GBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HT-29 using 83534-1-RR

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Gbp3 Recombinant Antibody (83534-1-RR, Clone:230178D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83534-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83534-1-RR against Human GBP3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, HUVEC-Zellen, Jurkat-Zellen, U-251-Zellen
• Erfolgreiche Detektion in FC (Intra): HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

83534-1-RR bindet in WB, FC (Intra), ELISA GBP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GBP3 fusion protein Ag33889
• Vollständiger Name: guanylate binding protein 3
• Beobachtetes Molekulargewicht: 62 kDa, 68 kDa
• GenBank-Zugangsnummer: BC140837
• Gene symbol: GBP3
• Gene ID (NCBI): 2635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.

Publikationen

Species	Application	Title
monkey	WB	J Virol; MA104 cell line is permissive for human bocavirus 1 infection; Authors - Jielin Tang