GCLC Polyklonaler Antikörper

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• KD/KO Validated

GCLC Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(147)

Rezensionen (2)

Kat-Nr. : 12601-1-AP

Synonyme

EC:6.3.2.2, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC

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  WB analysis using 12601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12601-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12601-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12601-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12601-1-AP

  IP result of anti-GCLC (IP:12601-1-AP, 4ug; Detection:12601-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug.

• 

  IHC staining of human kidney using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HEK-293-Zellen, Jurkat-Zellen, L02-Zellen, Mausnierengewebe, Mauslungengewebe, Raji-Zellen, Rattenlungengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 141 publications below
• IHC: See 7 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

12601-1-AP bindet in WB, IHC, IF, IP, ELISA GCLC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCLC fusion protein Ag3252
• Vollständiger Name: glutamate-cysteine ligase, catalytic subunit
• Berechnetes Molekulargewicht: 637 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC022487
• Gene symbol: GCLC
• Gene ID (NCBI): 2729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).

Publikationen

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang ; KO Validated
human	WB	Cell; Differential Requirements for eIF4E Dose in Normal Development and Cancer.; Authors - Morgan L Truitt
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Cell Metab; Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.; Authors - Joni Nikkanen
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zach (Verified Customer) (08-29-2025)

Our IHC core recommend me to get this antibody for IHC staining in human samples, pretty good experience.

• Applications: Western Blot, Immunohistochemistry
• Cell Tissue Type: pancreas, liver

FH

AIK SENG (Verified Customer) (01-10-2023)

Clear & crisp bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Colorectal cancer