GCLM Monoklonaler Antikörper

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• KD/KO Validated

GCLM Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1A5A4

Kat-Nr. : 66808-1-Ig

Synonyme

1A5A4, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GLCLR, Glutamate--cysteine ligase modifier subunit

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  WB analysis using 66808-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66808-1-Ig (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66808-1-Ig

  WB result of GCLM antibody (66808-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HEK-293 cells.

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  IHC staining of human liver cancer using 66808-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66808-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

66808-1-Ig bindet in WB, IHC, ELISA GCLM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GCLM fusion protein Ag5497
• Vollständiger Name: glutamate-cysteine ligase, modifier subunit
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC041809
• Gene symbol: GCLM
• Gene ID (NCBI): 2730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK.; Authors - Ning Ma
rat	WB	Eur J Med Chem; Design, synthesis and evaluation of fused hybrids with acetylcholinesterase inhibiting and Nrf2 activating functions for Alzheimer's disease; Authors - Yuanyuan Wang
human	WB	Front Cell Dev Biol; Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma; Authors - Feng Cao
human	WB	Mar Drugs; Fucoxanthin Attenuates Free Fatty Acid-Induced Nonalcoholic Fatty Liver Disease by Regulating Lipid Metabolism/Oxidative Stress/Inflammation via the AMPK/Nrf2/TLR4 Signaling Pathway; Authors - Jiena Ye