GDI1 Polyklonaler Antikörper

GDI1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10249-1-AP

Synonyme

GDI 1, GDI1, GDIL, GDP dissociation inhibitor 1, MRX41, MRX48, Oligophrenin 2, OPHN2, Protein XAP 4, Rab GDI alpha, RABGD1A, RABGDIA, XAP 4, XAP4

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10249-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10249-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10249-1-AP

  IP Result of anti-GDI1 (IP:10249-1-AP, 4ug; Detection:10249-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 4 publications below
• ELISA: See 1 publications below

Produktinformation

10249-1-AP bindet in WB, IP, IHC, IF, ELISA GDI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GDI1 fusion protein Ag0288
• Vollständiger Name: GDP dissociation inhibitor 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC000317
• Gene symbol: GDI1
• Gene ID (NCBI): 2664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55kda protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034) This antibody can bind both GDIs for the close sequences.

Publikationen

Species	Application	Title
human	WB,IF	Neurology; Antibodies in patients with neuropsychiatric systemic lupus erythematosus.; Authors - Kimura A A
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
mouse	WB	Neurobiol Dis; Disruption of Rab11 activity in a knock-in mouse model of Huntington's disease.; Authors - Xueyi Li
human,mouse	ELISA	Proteomics; 2-D DIGE analysis implicates cytoskeletal abnormalities in psychiatric disease.; Authors - English Jane A JA
mouse	IF	Int J Mol Sci; Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage; Authors - Masato Mashimo
human	IF	Nature; A cross-species proteomic map reveals neoteny of human synapse development; Authors - Li Wang