GFP tag Monoklonaler Antikörper

GFP tag Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

aequorea victoria, rekombinanten Protein

Anwendung

WB, IF, CoIP

Konjugation

HRP

Publikationen(19)

CloneNo.

1E10H7

Rezensionen (2)

Kat-Nr. : HRP-66002

Synonyme

eGFP, GFP, CFP, eYFP, GFP-tag

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  WB analysis of Recombinant protein using HRP-66002

  Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66002 (GFP tag Antibody) at dilution of 1:10000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

HRP-66002 bindet in WB, IF, CoIP GFP tag und zeigt Reaktivität mit aequorea victoria, rekombinanten Protein

• Getestete Reaktivität: aequorea victoria, rekombinanten Protein
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: GFP tag
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: -
• GenBank-Zugangsnummer: M62653
• Konjugation: HRP
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a HRP conjugated mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publikationen

Species	Application	Title
	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang
	WB,IF	PLoS Biol; Bridging integrator 1 fragment accelerates tau aggregation and propagation by enhancing clathrin-mediated endocytosis in mice; Authors - Xingyu Zhang
	WB	Mol Neurodegener; Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer's disease.; Authors - Guoxin Zhang
		Elife; A picorna-like virus suppresses the N-end rule pathway to inhibit apoptosis.; Authors - Zhaowei Wang
	WB	Aging Cell; Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease; Authors - Guoxin Zhang
	WB	Elife; Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.; Authors - Xingyu Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (09-11-2023)

It works well in our detection of GFP-tagged protein from Arabidopsis

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Arabidopsis

FH

Aamir (Verified Customer) (01-08-2020)

Worked fine for WB

• Applications: Western Blot,
• Primary Antibody Dilution: 10000
• Cell Tissue Type: HEks