GFP tag Monoklonaler Antikörper
GFP tag Monoklonal Antikörper für WB
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
aequorea victoria, rekombinanten Protein
Anwendung
WB, IF, CoIP
Konjugation
HRP Fluorescent Dye
CloneNo.
1E10H7
Kat-Nr. : HRP-66002
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Recombinant protein |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 7 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Produktinformation
HRP-66002 bindet in WB, IF, CoIP GFP tag und zeigt Reaktivität mit aequorea victoria, rekombinanten Protein
| Getestete Reaktivität | aequorea victoria, rekombinanten Protein |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Rekombinantes Protein |
| Vollständiger Name | GFP tag |
| Berechnetes Molekulargewicht | 26 kDa |
| Beobachtetes Molekulargewicht | - |
| GenBank-Zugangsnummer | U73901 |
| Gene symbol | |
| Gene ID (NCBI) | |
| Konjugation | HRP Fluorescent Dye |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3. |
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYFP tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. | ||
Mol Neurodegener Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer's disease. | ||
Elife Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. | ||
Plant Physiol Upstream Open Reading Frame and Phosphate-Regulated Expression of Rice OsNLA1 Controls Phosphate Transport and Reproduction. | ||
Front Microbiol The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Aamir (Verified Customer) (01-08-2020) | Worked fine for WB
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