GFPT2 Polyklonaler Antikörper

GFPT2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15189-1-AP

Synonyme

D-fructose-6-phosphate amidotransferase 2, EC:2.6.1.16, GFAT2, glutamine fructose 6 phosphate transaminase 2, Glutamine:fructose-6-phosphate amidotransferase 2

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  WB analysis of HT1080 cells using 15189-1-AP

  WB redult of GFPT2 antibody (15189-1-AP) with HT1080 cells.

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  IP experiment of HT-1080 using 15189-1-AP

  IP result of anti-GFPT2 (IP:15189-1-AP, 4ug; Detection:15189-1-AP 1:300) with HT-1080 cells lysate 2400ug.

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  IHC staining of human ovary cancer using 15189-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15189-1-AP (GFPT2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 15189-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15189-1-AP (GFPT2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT1080-Zellen
• Erfolgreiche IP: HT-1080.Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:500
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

15189-1-AP bindet in WB, IHC, IP, ELISA GFPT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFPT2 fusion protein Ag7275
• Vollständiger Name: glutamine-fructose-6-phosphate transaminase 2
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 75-77 kDa
• GenBank-Zugangsnummer: BC000012
• Gene symbol: GFPT2
• Gene ID (NCBI): 9945
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GFPT2 (Glutamine-fructose-6phosphate transaminase 2) is a rate-limiting enzyme in hexosamine biosynthesis involved in the occurrence and progress of many cancers (PMID: 35330716, 14764791). GFPT2 plays an important role in the metabolic activity of cells, especially in their glucose metabolism. GFPT2 expression was highly correlated with EMT-related factors (PMID: 37395335). High GFPT2 expression is associated with poor prognosis in various tumors, including lung adenocarcinoma, colorectal cancer, leiomyosarcoma, and serous ovarian cancer.

Publikationen

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	WB	Sci China Life Sci; Deamidation enables pathogenic SMAD6 variants to activate the BMP signaling pathway; Authors - Ling Li
mouse	WB	Prog Neuropsychopharmacol Biol Psychiatry; Dihydroartemisinin promotes tau O-GlcNAcylation and improves cognitive function in hTau transgenic mice; Authors - Lei Xia
		J Mol Cell Cardiol; A roadmap for the characterization of energy metabolism in human cardiomyocytes derived from induced pluripotent stem cells; Authors - Giulia Emanuelli