GGH Polyklonaler Antikörper

GGH Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 13264-1-AP

Synonyme

Conjugase, EC:3.4.19.9, Gamma-glutamyl hydrolase, Gamma-Glu-X carboxypeptidase, GH



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHL-60-Zellen, HepG2-Zellen, HT-1080.Zellen, MCF-7-Zellen
Erfolgreiche IPHepG2-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:5000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13264-1-AP bindet in WB, IHC, IF, IP, ELISA GGH und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GGH fusion protein Ag3939
Vollständiger Name gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
Berechnetes Molekulargewicht 318 aa, 36 kDa
Beobachtetes Molekulargewicht 30-37 kDa, 55 kDa
GenBank-ZugangsnummerBC025025
Gene symbol GGH
Gene ID (NCBI) 8836
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GGH (Gamma glutamyl hydrolase), also named as GH or Conjugase, is a key lysosomal enzyme involved in the metabolism of folic acid and in the action of antifolate drugs (PMID: 16945597). GGH catalyzes the removal of γ-linked polyglutamates from the intracellular folylpolyglutamates to yield folylmonoglutamate coenzymes (PMID: 9614206). The full-length protein has a calculated molecular mass of 36 kDa, contains four potential asparagine glycosylation sites, and was predicted to have a 24-amino-acid signal peptide (PMID: 8816764). GGH can form homodimer which contains two potential active sites (PMID: 16945597). Some bands can be detected by SDS-PAGE: 35-37kDa (full-length), 55kDa (glycosylated form) and 30-33kDa (signal peptide removed) (PMID: 8621474).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for GGH antibody 13264-1-APProtokoll herunterladen
IHC protocol for GGH antibody 13264-1-APProtokoll herunterladenl
IP protocol for GGH antibody 13264-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

J Clin Invest

Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer

Authors - Xiaochao Tan
WB

Front Pharmacol

Up-regulation of ABCG1 is associated with methotrexate resistance in acute lymphoblastic leukemia cells

Authors - Yao Chen
humanIHC,IF

Reprod Sci

The Identification of Gamma-Glutamyl Hydrolase in Uterine Corpus Endometrial Carcinoma: a Predictive Model and Machine Learning

Authors - Cheng Zhu
humanWB

Mol Med

Metformin reverses 5-FU resistance induced by radiotherapy through mediating folate metabolism in colorectal cancer

Authors - Shuxuan Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hua (Verified Customer) (04-04-2025)

Good antibody for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2, mouse liver, kidney, and brain
GGH Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, kidney, and brain (Cat no:13264-1-AP)