GHR Polyklonaler Antikörper

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• KD/KO Validated

GHR Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 20713-1-AP

Synonyme

GH binding protein, GH receptor, GHBP, GHR, Serum binding protein, Somatotropin receptor

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Galerie der Validierungsdaten

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  WB analysis of pig liver using 20713-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 20713-1-AP (GHR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of pig lung using 20713-1-AP

  pig lung tissue were subjected to SDS PAGE followed by western blot with 20713-1-AP (GHR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 20713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20713-1-AP (GHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 20713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20713-1-AP (GHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20713-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 20713-1-AP (GHR antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lebergewebe, Hausschwein-Lungengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below

Produktinformation

20713-1-AP bindet in WB, IHC, IF GHR und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GH binding protein
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_000163
• Gene symbol: GHR
• Gene ID (NCBI): 2690
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GH is a peptide hormone with many pleiotropic actions, including the regulation of metabolism, postnatal growth, cognition, immune, cardiac and renal systems and gut function (PMID: 26059750). GH exerts these actions primarily through alterations in gene expression, initiated by activation of its membrane receptor GHR and the resultant activation of the associated JAK2 and Src family kinases. GH receptor (GHR) is a member of the cytokine receptor family. Mutations in GHR gene have been associated with Laron syndrome, also known as the GH insensitivity syndrome (GHIS), a disorder characterized by short stature.

Publikationen

Species	Application	Title
human	WB	Pharmacol Res; Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin.; Authors - Long Wang
human	WB	J Cell Mol Med; GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling.; Authors - Hong-Zhu Yan ; KD Validated
human	WB	Cell Signal; The inhibition of GHR enhanced cytotoxic effects of etoposide on neuroblastoma.; Authors - Hongmei Zhang
human	WB	FEBS Open Bio; PMID: 31725956; Authors - Mo Cheng ; KD Validated
human	WB	FEBS Open Bio; PMID: 32069380; Authors - Xiaojue Zhu ; KD Validated