GIPC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GIPC1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Rezensionen (2)

Kat-Nr. : 14822-1-AP

Synonyme

C19orf3, GAIP C-terminus-interacting protein, GIPC, PDZ domain-containing protein GIPC1, RGS19-interacting protein 1

---

• 

  WB analysis using 14822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14822-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14822-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14822-1-AP

  IP result of anti-GIPC1 (IP:14822-1-AP, 4ug; Detection:14822-1-AP 1:500) with mouse brain tissue lysate 9500ug.

• 

  IHC staining of human breast cancer using 14822-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14822-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14822-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GIPC1 antibody (14822-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of A2780 using 14822-1-AP

  Immunofluorescent analysis of A2780 cells, using 14822-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A2780-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:600-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

14822-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GIPC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GIPC1 fusion protein Ag6597
• Vollständiger Name: GIPC PDZ domain containing family, member 1
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC000410
• Gene symbol: GIPC1
• Gene ID (NCBI): 10755
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAIP interacting protein C terminus, member 1(GIPC1), also known as PDZ domain-containing protein GIPC1, is a cytoplasmic protein that also localizes to the peripheral membrane, acting as an adaptor protein linking receptor interactions to intracellular signaling pathways, including cell cycle regulation, and expression has been associated with some cancers (PMID: 19175293, 26757732). This antibody is specific to GIPC1 with a band at 42 kDa (PMID: 18721486).

Publikationen

Species	Application	Title
human	IF	Am J Hum Genet; Expansion of GGC Repeat in GIPC1 Is Associated with Oculopharyngodistal Myopathy.; Authors - Jianwen Deng
human	IHC	Dev Cell; SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt.; Authors - Christoph J Burckhardt
human	WB	Ann Neurol; GIPC1 CGG repeat expansion is associated with movement disorders.; Authors - Yu Fan
mouse	WB	Pain; G protein-coupled receptor GPR151 is involved in trigeminal neuropathic pain through the induction of Gβγ/extracellular signal-regulated kinase-mediated neuroinflammation in the trigeminal ganglion.; Authors - Bao-Chun Jiang
mouse	WB	FASEB J; BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.; Authors - Nahoko Eiraku ; KD Validated
human,mouse	WB	CNS Neurosci Ther; G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7.; Authors - Yong Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tom (Verified Customer) (11-02-2023)

Used at 1:2000 on PVDF membrane, overnight incubation at 4C in 3% BSA PBS 0.1% Tween. No non-specific bands and band reduced by knockdown with GIPC1 siRNA. Diluted antibody succesfully re0used after freezing at -20C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HUVEC

GIPC1 Antibody Western Blot validation (1:2000 dilution) in HUVEC (Cat no:14822-1-AP)

FH

Sophy (Verified Customer) (01-27-2023)

I ordered a GIPC1 antibody from another company before and the results were poor. Proteintech antibody worked very well with the dilution of 1:1000 in western.

• Applications: Western Blot
• Cell Tissue Type: NSCLC cells