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GIT1 Polyklonaler Antikörper

GIT1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26247-1-AP

Synonyme

Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, CAT 1, ARF GTPase-activating protein GIT1

Filter:
  • Western Blot (WB) analysis of ROS1728 cells using GIT1 Polyclonal antibody (26247-1-AP)

    WB analysis of ROS1728 using 26247-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 26247-1-AP (GIT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBROS1728-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

26247-1-AP bindet in WB, ELISA GIT1 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GIT1 fusion protein Ag24648
Vollständiger Name G protein-coupled receptor kinase interacting ArfGAP 1
Berechnetes Molekulargewicht 694 aa, 77 kDa
Beobachtetes Molekulargewicht80 kDa
GenBank-ZugangsnummerBC067358
Gene symbol GIT1
Gene ID (NCBI) 28964
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GIT1 (ARF GTPase-activating protein GIT1) is a member of the GIT subfamily of ADP ribosylation factor (ARF)-GTPase activating protein (GAP) family of proteins, which are characterized by an ARFGAP domain that stimulates the GTPase activity of proteins. GIT1 was highly expressed in the nervous system, and its expression was maintained throughout all brain neuritogenesis stages (PMID: 27127481).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for GIT1 antibody 26247-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Sammy (Verified Customer) (11-20-2023)

Detects and band above the expected kDa but has been validated by knock down. Antibody also works by immunofluorescence where it detects GIT1 at focal adhesions.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000
  • Cell Tissue Type: HUVEC
GIT1 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:26247-1-AP)