Connexin-43 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Connexin-43 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(35)

Kat-Nr. : 15386-1-AP

Synonyme

GJA1, Connexin 43, GJAL, Gap junction alpha-1 protein, Gap junction alpha 1 protein

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  WB analysis of HeLa using 15386-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15386-1-AP (GJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15386-1-AP

  IP result of anti-Connexin-43 (IP:15386-1-AP, 4ug; Detection:15386-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of mouse heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse heart using 15386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15386-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 4 publications below
• IF: See 17 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15386-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA Connexin-43 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Connexin-43 fusion protein Ag6698
• Vollständiger Name: gap junction protein, alpha 1, 43kDa
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43-47 kDa
• GenBank-Zugangsnummer: BC026329
• Gene symbol: Connexin-43
• Gene ID (NCBI): 2697
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Connexin-43 (Cx43, also known as gap junction alpha-1), a connexin family member, plays an essential role in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

Publikationen

Species	Application	Title
human	IF	Nat Commun; Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation.; Authors - Yin Wang
mouse	IF	Thromb Res; Selective increase of cardiomyocyte derived extracellular vesicles after experimental myocardial infarction and functional effects on the endothelium.; Authors - Jose A Rodriguez
mouse	WB	Hypertension; Novel Role for the Immunoproteasome Subunit PSMB10 in Angiotensin II-Induced Atrial Fibrillation in Mice.; Authors - Jing Li
	IF	Ecotoxicol Environ Saf; Sodium Selenite inhibits mitophagy, downregulation and mislocalization of blood-testis barrier proteins of bovine Sertoli cell exposed to microcystin-leucine arginine (MC-LR) via TLR4/NF-kB and mitochondrial signaling pathways blockage.; Authors - E O Adegoke
human	WB	Environ Toxicol Pharmacol; Microcystin-leucine arginine (MC-LR) induced inflammatory response in bovine sertoli cell via TLR4/NF-kB signaling pathway.; Authors - E O Adegoke
human	IF	Free Radic Biol Med; Oxidative stress contributes to hepatocyte growth factor-dependent pro-senescence activity of ovarian cancer cells.; Authors - Justyna Mikuła-Pietrasik