Glycerokinase Polyklonaler Antikörper

Glycerokinase Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13360-1-AP

Synonyme

GK, GK1, GKD, Glycerokinase, glycerol kinase

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 13360-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13360-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13360-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13360-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13360-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13360-1-AP

  IP Result of anti-Glycerokinase (IP:13360-1-AP, 4ug; Detection:13360-1-AP 1:1000) with HepG2 cells lysate 4000ug.

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  IF Staining of HepG2 using 13360-1-AP

  Immunofluorescent analysis of HepG2 cells using 13360-1-AP (Glycerokinase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushodengewebe, Mauslebergewebe, Mausnierengewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

13360-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA Glycerokinase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Glycerokinase fusion protein Ag4175
• Vollständiger Name: glycerol kinase
• Berechnetes Molekulargewicht: 524 aa, 57 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC037549
• Gene symbol: GK
• Gene ID (NCBI): 2710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu
mouse	WB	J Appl Toxicol; Aberrant expression of miR-451a contributes to 1,2-dichloroethane-induced hepatic glycerol gluconeogenesis disorder by inhibiting glycerol kinase expression in NIH Swiss mice.; Authors - Ni Zeng
human	WB	Life Sci Alliance; Serine palmitoyltransferase assembles at ER-mitochondria contact sites.; Authors - Mari J Aaltonen
human,mouse	IHC	J Adv Res; O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation.; Authors - Hongshuo Zhang
human,mouse	WB,IHC	Mol Metab; The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326; Authors - Brian E Ford
mouse	IF,RIP	Arterioscler Thromb Vasc Biol; Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice; Authors - Cui Ma