GLAST Polyklonaler Antikörper

GLAST Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (2)

Kat-Nr. : 20785-1-AP

Synonyme

EA6, EAAT1, GLAST, GLAST 1, GLAST1, SLC1A3

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Galerie der Validierungsdaten

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  WB analysis of Neuro-2a using 20785-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 20785-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20785-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20785-1-AP

  IP Result of anti-GLAST (IP:20785-1-AP, 4ug; Detection:20785-1-AP 1:500) with mouse brain tissue lysate 3000ug.

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  IHC staining of human brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of Neuro-2a using 20785-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using GLAST antibody (20785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Neuro-2a using 20785-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human GLAST (20785-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, C6-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Neuro-2a-Zellen
• Erfolgreiche Detektion in FC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

20785-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA GLAST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Kuh
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLAST fusion protein Ag14177
• Vollständiger Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• Berechnetes Molekulargewicht: 542 aa, 60 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 90-100 kDa
• GenBank-Zugangsnummer: BC037310
• Gene symbol: SLC1A3
• Gene ID (NCBI): 6507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)

Publikationen

Species	Application	Title
mouse	IF	Nucleic Acids Res; H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.; Authors - Libo Su
mouse		Cell Rep; SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice; Authors - Yan Wang
mouse	WB, IF	Cell Death Differ; RNF20 controls astrocytic differentiation through epigenetic regulation of STAT3 in the developing brain.; Authors - Qingli Liang
mouse	WB	Neuropharmacology; Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease.; Authors - Yun-Long Zhang
mouse	WB	Sci Rep; Impact of Reduced Cerebellar EAAT Expression on Purkinje Cell Firing Pattern of NPC1-deficient Mice.; Authors - Michael Rabenstein
mouse	WB	J Pers Med; Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling; Authors - Qian Bai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (02-14-2018)

NaCit antigen retrieval ph = 6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina and brain at 5dpf

GLAST Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish retina and brain at 5dpf (Cat no:20785-1-AP)

FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - 0.1M Tris-HCl ph9

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina/brain

GLAST Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish retina/brain (Cat no:20785-1-AP)