GLAST Polyklonaler Antikörper

GLAST Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-20785

Synonyme

EA6, EAAT1, FLJ25094, GLAST, GLAST 1, GLAST1, SLC1A3

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Galerie der Validierungsdaten

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  IF Staining of Neuro-2a using CL488-20785

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CoraLite® Plus 488 GLAST antibody (CL488-20785) at dilution of 1:200.

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  FC experiment of Neuro-2a using CL488-20785

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GLAST (CL488-20785) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Neuro-2a-Zellen
• Erfolgreiche Detektion in FC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-20785 bindet in IF, FC (Intra) GLAST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLAST fusion protein Ag14177
• Vollständiger Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• Berechnetes Molekulargewicht: 542 aa, 60 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 90-100 kDa
• GenBank-Zugangsnummer: BC037310
• Gene symbol: SLC1A3
• Gene ID (NCBI): 6507
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)