GLI3 Polyklonaler Antikörper

GLI3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 28272-1-AP

Synonyme

GLI3-190, GLI3 full-length protein, GLI3 form of 190 kDa, GLI family zinc finger 3, GCPS

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  WB analysis of HepG2 using 28272-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28272-1-AP (GLI3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 28272-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 28272-1-AP (GLI3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

28272-1-AP bindet in WB, IHC, ELISA GLI3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLI3 fusion protein Ag28472
• Vollständiger Name: GLI family zinc finger 3
• Berechnetes Molekulargewicht: 170 aa
• Beobachtetes Molekulargewicht: 170-190 kDa, 80-90 kDa
• GenBank-Zugangsnummer: NM_000168
• Gene symbol: GLI3
• Gene ID (NCBI): 2737
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

Publikationen

Species	Application	Title
rat	WB	Bioengineered; All-trans-retinoic acid suppresses rat embryo hindlimb bud mesenchymal chondrogenesis by modulating HoxD9 expression; Authors - Quan Hong
human	WB	Medicine (Baltimore); miR-200c targeting GLI3 inhibits cell proliferation and promotes apoptosis in non-small cell lung cancer cells; Authors - Xiangjun Yi