GLIS3 Polyklonaler Antikörper

GLIS3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12678-1-AP

Synonyme

GLI similar 3, GLIS family zinc finger 3, GLIS3, Zinc finger protein 515, Zinc finger protein GLIS3, ZNF515

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 12678-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12678-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 12678-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12678-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, A549-Zellen, BxPC-3-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12678-1-AP bindet in WB, ELISA GLIS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLIS3 fusion protein Ag3366
• Vollständiger Name: GLIS family zinc finger 3
• Berechnetes Molekulargewicht: 775 aa, 84 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC033899
• Gene symbol: GLIS3
• Gene ID (NCBI): 169792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. GLIS3 (gLIS family zinc finger 3), also known as ZNF515 that localizes to the nucleus and contains five C2H2-type zinc fingers. Expressed in a variety of tissues, including kidney, brain, liver, lung, ovary, pancreas, thymus and skeletal muscle, GLIS3 functions as both an activator and a suppressor of transcription, specifically binding the consensus sequence 5'-GACCACCCAC-3' through its C2H2-type zinc fingers. Defects in the gene encoding GLIS3 are a cause of NDH syndrome; a neonatal diabetes that is characterized by congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. There are various isoform of GLIS3 and molecular weight of one isoform is 70 kDa.

Publikationen

Species	Application	Title
mouse	WB	Physiol Genomics; Polycystin-2-dependent transcriptome reveals early response of autosomal dominant polycystic kidney disease; Authors - Hyun Jun Jung