KGA/GAC Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

KGA/GAC Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

3A12A1

Rezensionen (2)

Kat-Nr. : 66265-1-Ig

Synonyme

AAD20, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA, KIAA0838, L glutamine amidohydrolase

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 66265-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66265-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66265-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66265-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66265-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 66265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66265-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66265-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 66265-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using KGA/GAC antibody (66265-1-Ig, Clone: 3A12A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of mouse brain using 66265-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66265-1-Ig (GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66265-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66265-1-Ig(GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen, Rattenlebergewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, HepG2-Zellen, L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

66265-1-Ig bindet in WB, IHC, IF, ELISA KGA/GAC und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KGA/GAC fusion protein Ag24227
• Vollständiger Name: glutaminase
• Berechnetes Molekulargewicht: 669 aa, 73 kDa
• Beobachtetes Molekulargewicht: 58 kDa, 65 kDa
• GenBank-Zugangsnummer: BC038507
• Gene symbol: GLS
• Gene ID (NCBI): 2744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.

Publikationen

Species	Application	Title
		J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
human	IHC,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB,IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KO Validated
mouse	WB	Cell Rep; Glutaminase 1 deficiency confined in forebrain neurons causes autism spectrum disorder-like behaviors; Authors - Chenhui Ji
mouse	IF	Oxid Med Cell Longev; Pharmacological Inhibition of Glutaminase 1 Attenuates Alkali-Induced Corneal Neovascularization by Modulating Macrophages.; Authors - Yifan Feng
human	WB	Int J Oncol; Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells.; Authors - Tokio Terado

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works well

• Applications: Western Blot

FH

Longlong (Verified Customer) (03-01-2022)

I'm working on a glutaminase project. Great western blot results and fantastic consistency

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human AML cell lines

KGA/GAC Antibody Western Blot validation (1:1000 dilution) in human AML cell lines (Cat no:66265-1-Ig)