GLT25D1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GLT25D1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 16768-1-AP

Synonyme

PSEC0241, EC:2.4.1.50, Collagen beta(1-O)galactosyltransferase 1, COLGALT1, ColGalT 1

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  WB analysis of SH-SY5Y using 16768-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 16768-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 16768-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 16768-1-AP

  IP result of anti-GLT25D1 (IP:16768-1-AP, 4ug; Detection:16768-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human ovary tumor using 16768-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16768-1-AP (GLT25D1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 16768-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 16768-1-AP (GLT25D1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 16768-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 16768-1-AP (GLT25D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 16768-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 16768-1-AP (GLT25D1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, Maus-Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

16768-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GLT25D1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLT25D1 fusion protein Ag10346
• Vollständiger Name: glycosyltransferase 25 domain containing 1
• Berechnetes Molekulargewicht: 231 aa, 27 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC020492
• Gene symbol: GLT25D1
• Gene ID (NCBI): 79709
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Ann Neurol; Biallelic COLGALT1 variants are associated with cerebral small vessel disease.; Authors - Satoko Miyatake
mouse,rat	WB	J Mol Cell Cardiol; Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.; Authors - Randy T Cowling
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
mouse	WB	Sci Rep; Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes; Authors - Masahiko Terajima
human	WB	Biochemistry; Role of Glycosyltransferase 25 domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes.; Authors - Masahiko Terajima ; KD Validated
mouse	WB,IHC	Mol Med Rep; Downregulation of Glt25d1 aggravates carbon tetrachloride‑induced acute hepatic injury through activation of the TGF‑β1/Smad2 signaling pathway.; Authors - Xiaohui Ye ; KO Validated