• Featured Product
  • KD/KO Validated

GOLGA2/GM130 Monoklonaler Antikörper

GOLGA2/GM130 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

CloneNo.

2A4F11

Kat-Nr. : 66662-1-Ig

Synonyme

GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, 2A4F11, GM130 autoantigen



Geprüfte Anwendungen

Erfolgreiche Detektion in WBLNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, PC-12-Zellen
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66662-1-Ig bindet in WB, IF/ICC, ELISA GOLGA2/GM130 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen GOLGA2/GM130 fusion protein Ag1848
Vollständiger Name golgi autoantigen, golgin subfamily a, 2
Berechnetes Molekulargewicht 111 kDa
Beobachtetes Molekulargewicht 130 kDa
GenBank-ZugangsnummerBC014188
Gene symbol GM130
Gene ID (NCBI) 2801
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for GOLGA2/GM130 antibody 66662-1-IgProtokoll herunterladen
IF protocol for GOLGA2/GM130 antibody 66662-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu
humanWB

Mol Metab

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Authors - Han Zeng
rat,humanWB

Front Mol Biosci

Schwann Cell-Derived Exosomes Induce the Differentiation of Human Adipose-Derived Stem Cells Into Schwann Cells.

Authors - Nan Zhou
humanIF

Exp Cell Res

NLRX1 and STING alleviate renal ischemia-reperfusion injury by regulating LC3 lipidation during mitophagy

Authors - Yinping Liao
mouseWB

STAR Protoc

Protocol for extraction of gut interstitial fluid in mice with two-front nutrient supply

Authors - Jian Zhang
humanIF

bioRxiv

SPINK1-COL18A1 Crosstalk Shapes Epigenome and Drives Cancer Stemness in Pancreatic Ductal Adenocarcinoma

Authors - Haoyu Tang