GMF Beta Monoklonaler Antikörper

GMF Beta Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

3B10A2

Kat-Nr. : 60062-1-Ig

Synonyme

glia maturation factor beta, GMF Beta, GMFB, GMF-beta

---

• 

  WB analysis of fetal human brain using 60062-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 60062-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 60062-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 60062-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of U-87 MG using 60062-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using GMF Beta antibody (60062-1-Ig, Clone: 3B10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60062-1-Ig

  Immunofluorescent analysis of Hela cells, using GMFB antibody 60062-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Hausschwein-Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-87 MG-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

60062-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA GMF Beta und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GMF Beta fusion protein Ag1104
• Vollständiger Name: glia maturation factor, beta
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC005359
• Gene symbol: GMFB
• Gene ID (NCBI): 2764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.

Publikationen

Species	Application	Title
human	IF	Int J Biol Macromol; SUMOylation of GMFB regulates its stability and function in retinal pigment epithelial cells under hyperglycemia; Authors - Wan Sun
human	IP	Transl Psychiatry; Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein.; Authors - Brawnie Petrov
human	IF	Front Oncol; Glia Maturation Factor β as a Novel Independent Prognostic Biomarker and Potential Therapeutic Target of Kidney Renal Clear Cell Carcinoma.; Authors - Tong Zhu
human	IF	J Alzheimers Dis; Co-Localization of Glia Maturation Factor with NLRP3 Inflammasome and Autophagosome Markers in Human Alzheimer's Disease Brain.; Authors - Mohammad Ejaz Ahmed
human, mouse	IF	PLoS One; Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.; Authors - Duraisamy Kempuraj